Conceptual and methodological advances in cell-free directed evolution
نویسندگان
چکیده
منابع مشابه
Advances in the directed evolution of proteins.
Natural evolution has produced a great diversity of proteins that can be harnessed for numerous applications in biotechnology and pharmaceutical science. Commonly, specific applications require proteins to be tailored by protein engineering. Directed evolution is a type of protein engineering that yields proteins with the desired properties under well-defined conditions and in a practical time ...
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Naturally occurring enzymes are remarkable biocatalysts with numerous potential applications in industry and medicine. However, many of their catalyst properties often need to be further tailored to meet the specific requirements of a given application. Within this context, directed evolution has emerged over the past decade as a powerful tool for engineering enzymes with new or improved functi...
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1Department of Integrative Biology and Museum of Vertebrate Zoology, University of California, Berkeley CA 94720 USA; and 2Department of Ecology and Evolutionary Biology, University of California at Los Angeles, 651 Charles Young Dr. South, Los Angeles, CA 90095, USA; *Correspondence to be sent to: Department of Integrative Biology, University of California, Berkeley CA 94720, USA; Email: drabo...
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1Department of Physics and BIOtech, University of Trento, 38123 Mattarello, Italy 2Department of Information and Communication Technologies, Universitat Pompeu Fabra, 08018 Barcelona, Spain 3 J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611-6131, USA 4Department of Mathematical, Physical and Computational Sciences, Faculty of Engineerin...
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ژورنال
عنوان ژورنال: Current Opinion in Structural Biology
سال: 2015
ISSN: 0959-440X
DOI: 10.1016/j.sbi.2015.04.008